The Lusaka Water and Sewerage Company (LWSC) produces ~800–1,000 kg of treated sewage sludge per day at its Manchinchi wastewater treatment plant (WWTP). The biosolids are used for land application purposes although the contaminant and pathogen composition and quality of the biosolids have been unknown until this study. Zambia does not have legal standards and guidelines for biosolids management or application. The Manchinchi plant in Lusaka suffers from constant breakdowns such that the effectiveness of the plant to produce quality grade biosolids for land application use is questionable. In peri-urban areas, the problem of poor sanitation is being addressed using different technologies including urine diversion ecosan toilets. The effectiveness of ecosan toilets to stabilize faecal sludge has not been assessed in Zambia. The purpose of this study was to stabilize and characterize the biosolids from Manchinchi plant and ecosan toilets. Stabilization was done by use of drying beds and irradiation. The parametres that were used for characterization were microbiological, parasitological and heavy metals.